Methods Epithelial cells were isolated from sample tissue by cold trypsinization and cultured in serum-free growth medium.
方法 标本组织消化法分离出尿路上皮细胞,于无血清生长基。
Methods Epithelial cells were isolated from sample tissue by cold trypsinization and cultured in serum-free growth medium.
方法 标本组织消化法分离出尿路上皮细胞,于无血清生长基。
Methods Separate the embryonic fibroblast of mice and culture with the method of suspension culture and method of trypsinization.
方法取大鼠胚分离成纤维细胞,分别采细胞悬液法和胰酶消化植法对其进。
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