After absorption, prulifloxacin is metabolised by esterases to ulifloxacin, which inhibits the formation of superhelix, cell division and reproduction by interfering the DNA toposomerase.

本为前体药,其活性代谢产物通过阻碍DNA拓扑构酶使细菌DNA法形成超螺旋,导致细菌细法分裂繁殖。