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Result Both mutanolysin and lysozyme could successfully lyse the wall of bifidobacterium and extract the plasmid from Bifidobacterium.

球孢链霉菌发酵液与溶菌酶均成功提取到质粒。

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Method Bifidobacterium plasmid was extracted by mutanolysin from Streptomyces globisporus which could lyse the wall of bifidobacterium.The result was compared with that by lysozyme.

法利用球孢链霉菌发酵产生的变溶菌素来消化菌细胞壁,提取质粒,并与商业化溶菌酶法比较。

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Some are exchanged via small loops of DNA called plasmids.

一些基因会通被称为的DNA小环交换。

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Osmosis-微生物

Additionally to the genetic material contained within the nucleoid, many bacteria contain extra chromosomal DNA molecules called plasmids.

除了包含在拟中的遗传物外,许多菌含有额外的DNA分子,称为

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Did You Know? – Verge Science

Then they dropped the plasmid into a living bacterium, or yeast.

然后们将放入活菌或酵母中。

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Osmosis-微生物

However, plasmids often contain genes that confer a selective advantage to the bacterium such as resistance to antibiotics.

通常包含赋予菌选择性优势的基因,例如抗生素耐药性。

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Osmosis-微生物

These plasmids are small double-stranded DNA molecules that can exist independently of the chromosome and they can be circular or linear.

它们是双链的DNA小分子,可以独立于菌染色体(拟)而存在,可以是环状或线状的。

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2017级专外有声

Transfer of the ligated plasmid into an E. coli strain (transformation).

7。转化成功的菌的筛选。

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Life Noggin

The plasmid enters and multiplies itself within a cell and can then be transferred to others.

进入胞并在胞内繁殖, 然后可以转移到胞中。

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Life Noggin

So essentially, these plasmids are the vehicles that transfer genes for antibiotic resistance between different bacterial species.

因此本上,这些是在不同菌物种之间转移抗生素抗性基因的载体。

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SciShow 合集

This plasmid mutation has been seen in other places before; in China, in animals and some human patients.

这种突变以前在地方也见;在中国,在动物和一些人类患者中。

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2017级专外有声

The cDNAs had to be linked to prokaryotic transcription and translation signals and inserted into multicopy plasmids.

然而,占总蛋白30%的量表达的蛋白是沉淀或不溶的形式并且缺少翻译后修饰。

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Osmosis-微生物

Plasmids have relatively few genes and their genetic information is not essential to the bacterium and cells that lack them usually function normally.

所含的基因相对较少,并且它们的基因对菌来说并不是必须的,缺少胞往往也可以正常生长。

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SciShow 合集

But this is the first time the plasmid has been found in the United States, and it could get passed around quickly.

但这是第一次在美国发现这种,它可能会很快传播开来。

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2017级专外有声

More specialized vectors have been engineered to use aspects of plasmids and bacteriophages, such as the plasmid-bacteriophage h hybrids known as cosmids

和噬菌体已经在很多种类的菌中被使用来表达基因,并且一些噬菌体也许已经被用来将DNA整合到宿主基因组上。

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2017级专外有声

Phage h can be used to clone fragments larger than plasmid vectors, and phage M13 allows cloned DNA to be isolated in single-stranded form.

更多载体被改造能同时拥有和噬菌体的一些特性,像被称为粘-噬菌体λ杂交体。

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Life Noggin

Or, in plain english, plasmids are part of the reason that, one day, antibiotics may not be able to help your strep throat anymore.

或者,用简单的英语来说,是有一天抗生素可能无法再帮助您治疗链球菌性咽喉炎的部分原因。

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2022诺贝尔得主访谈录

I did all these experiments actually, with my own hands, I was 58 years old, I was still culturing plasmids and feeding cells, so it is very unlikely.

实际上,所有这些实验都是我亲手做的,当时我 58 岁,我还在培养和喂养胞,所以这是非常不可能的。

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Osmosis-微生物

The cytoplasm contains ribosomes which are the site of protein synthesis, the nucleoid which contains the genetic material, and plasmids which contain genes that confer a selective advantage to the bacterium.

中含有糖体,它们是蛋白合成的部位;拟含有遗传物以及含有赋予菌选择性优势的基因。

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Life Noggin

But also, a much more terrifying explanation has to do with plasmids, which are parts of the bacterial DNA. The plasmid enters and multiplies itself within a cell and can then be transferred to others.

而且,一个更可怕的解释与有关,菌 DNA 的一部分。 进入胞并在胞内自我繁殖, 然后可以转移到胞中。

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SciShow 合集

And the thing that makes this mutation especially troubling is that it was found on a plasmid -- a little piece of DNA that can be easily shared between neighboring bacteria, meaning it can spread more easily.

让这种突变特别麻烦的是它是在上发现的——一小段 DNA 可以很容易地在相邻菌之间共享,这意味着它可以更容易地传播。

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