Taq polymerase is used to amplify the cDNA through denaturation, annealing and extension steps.

Taq聚合通过变、退火、延伸等步骤用于扩增cDNA。

The first step is denaturation, then there's annealing, and finally extension.

第一步是变，是退火，最是延伸。

So not only can Taq polymerase withstand the heat during the denaturation step of PCR, but it also functions best at around 72 degrees Celsius.

因此，Taq聚合不仅能承受PCR变步骤中的热量，而72氏度左右能发挥出最好的作用。

These alcohols can react with different parts of many proteins, disrupting the bonds that hold them together, and ultimately breaking them down in a process known as denaturation.

这些酒精可以和很多蛋白质的不同部分产生反应，破坏将它们结合一起的化学键，并最终将其分解，这一过程称为变。