The expression product of ScFv was purified through Ni-NTA agarose metal affinity resin column and renatured.
经合亲和层析柱(Ni-NTA agarose)纯化和凝胶柱上在位复性后,ELISA结果证实其具有良好的结合β3抗原性。
TEM, ethidium bromide displacement assays, agarose gel electrophoresis and SAXS studies support the formation of lipoplexes for the transfection of CHO cells.
【文献摘要】A new transfection reagent based on nucleoside phosphocholine amphiphile leading to high transfection efficacy and low cytotoxicity is described.
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