The expression product of ScFv was purified through Ni-NTA agarose metal affinity resin column and renatured.

经金属螯合亲和层析柱(Ni-NTA agarose)纯化和凝胶柱上在位复性，ELISA证实其具有良好的合β3抗原性。

Tigogenin was separated completely from the crude sapogenins by macroporous resin column chromatography,only simple solvent Ilelute was used in the column of the separate process.

用大孔树脂为吸附剂的色法在分离的过程中仅需要简单的溶剂-Ⅱ,分离出的单一组分剑麻皂甙元占粗皂甙元的比率高达70%以上。